METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 5

MiR-30b-3p inhibited osteogenic differentiation of pPDLSCs: (a) the protein level of Runx2 was determined by western blotting at 7 days of osteogenic induction transfection with miR-30b-3p mimic and inhibitor; (b) the mRNA levels of ALP, Runx2, and Col1 were detected at 7 days of osteogenic induction after transfection with miR-30b-3p mimic and inhibitor; (c) osteogenic differentiation was detected by ALP staining at 7 days of osteogenic induction after transfection with miR-30b-3p mimic and inhibitor; (d) correlation analysis between miR-30b-3p levels and Runx2 levels in pPDLSCs 0, 1, 7, and 14 days after osteogenic induction. Significance levels were determined by () , () , and () .

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