METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 6

LncRNA CUTALP modulated Runx2 through regulation of miR-30b-3p: (a) the protein level of Runx2 was detected by western blotting after osteogenic differentiation in pPDLSCs; (b) qRT-PCR was used to detect the expression changes of Runx2 with osteogenic induction; (c) the mRNA levels of Runx2 were detected by qRT-PCR after transfection with miR-30b-3p mimic and inhibitor; (d) the protein levels of Runx2 were detected by western blotting after transfection with miR-30b-3p mimic and inhibitor; (e) the mRNA levels of Runx2 were detected by qRT-PCR after lncRNA CUTALP overexpression and knockdown; (f) the protein levels of Runx2 were detected by western blotting after ncRNA CUTALP overexpression and knockdown; (g and h) qRT-PCR and western blotting showed that the inhibition of osteogenic markers caused by lncRNA CUTALP knockdown in pPDLSCs would be recovered by miR-30b-3p inhibitor. Significance levels were determined by () , () , () , and () .

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