Improving Cutaneous Wound Healing in Diabetic Mice Using Naturally Derived Tissue-Engineered Biological Dressings Produced under Serum-Free Conditions

Figure 5

NONcNZO/LtJ10 mice treated wounds display higher collagen content with balanced collagen I/III proportions. Representative images of the (a) untreated and (b) dressing-treated wound/healed skin visualized after Picrosirius red staining of paraffin-embedded tissue sections on day 20 after surgery. Bars: 500 µm. The wound area is indicated by the white arrowheads, and areas used for quantification are illustrated by the white dotted rectangles. (a′–d′) Magnifications of the areas delimited by the white boxes (a–d) shown in the complete tissue sections are presented on the right. Bars: 100 µm. (c) percentage of the neodermis area occupied by collagen fibers. n = 6–7 wounds/group, mean ± SD, unpaired t-test with Welch’s correction,  : . (d) The proportion of green-, yellow-, and red-colored collagen fibers after subtraction of unoccupied space (black color). n = 6–7 wounds/group, mean ± SD, one-way ANOVA with Tukey’s post-hoc test. No statistically significant differences were observed between the two groups for each color (green, yellow, red).

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