Effects of Extracellular Vesicles Derived from Human Umbilical Cord Blood Mesenchymal Stem Cells on Cell Immunity in Nonobese Mice

Figure 2

Characteristics of HucMSC and MSC-derived EVs. Adherent cells isolated from umbilical cord showed a fibroblast-like morphology. (a) Osteogenic differentiation was demonstrated by the intracytoplasmic accumulation of alkaline phosphatase. Lipid-rich drops stained with oil red O indicated adipogenesis differentiation ability. HucMSC were characterized by flow cytometry. (b) Cells were negative for CD45 and CD34, while they were positive for CD29 and CD90. (c) Morphology of MSCs-EVs under a transmission electron microscopy. (d) By Western blot analysis, specific markers for EVs (CD9, CD63, and CD81) tested positive, while calnexin tested negative. (e) NTA analysis indicated that 92.6% of MSC-derived EVs size were in the size ranged 132–175 nm, and the average concentration of HucMSC-EVs particles measured was 2.2E + 10 particles/ml.

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