TLR3 Agonist Amplifies the Anti-Inflammatory Potency of ADSCs via IL-10-Mediated Macrophage Polarization in Acute Pancreatitis

Figure 2

The anti-inflammatory properties of poly(I : C) treated ADSCs: (a) the representative morphology of ADSCs with 10–50 μg/mL poly(I : C) for 24 hr (Bar = 200 μm), (b) the poly(I : C) stimulation activated the expression of TLR3, detected by immunofluorescence (green: TLR3, blue: DAPI to indicate the nucleus; n = 3; Bar = 50 μm), (c) the anti-inflammatory or proinflammatory cytokines gene expression with poly(I : C) stimulation (n = 6), (d) the levels of IL-6, IL-10, TNF-α, and TGF-β in poly(I : C) stimulated ADSCs culture supernatant, (e) the migration of 25 μg/mL poly(I : C) treated ADSCs (n = 8) and (f, g) The homing capacity of ADSCs in taurocholate-induced pancreatitis mouse pancreas and lung after 24 hr tail vein injection with 2.5 × 10⁵ per mice (red: Dil-labeled ADSCs, blue: DAPI to indicate the nucleus; n = 6; Bar = 50 μm). The data were represented as mean ± SD, the post hoc Tukey correction was performed after ANOVA was used for comparison of more than two groups. , .

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