TLR3 Agonist Amplifies the Anti-Inflammatory Potency of ADSCs via IL-10-Mediated Macrophage Polarization in Acute Pancreatitis

Figure 7

The poly(I : C) treated ADSCs with superior effects in skewing macrophage into anti-inflammatory phenotype: (a) the classically or alternatively activated macrophages were representative of CD86 and CD206 using a flow cytometry assay (n = 3), (b) the mRNA expression of IL-1β, IL-6, TNF-α, and IL-10 in cocultured macrophage (n = 6), (c) the protein expression of JAK2 and STAT3 in cocultured macrophage (n = 3), (d) the infiltration of macrophage (F4/80), (e) neutrophils (MPO; red: F4/80 or MPO positive cell, blue: DAPI to indicate the nucleus), and (f) polarization of macrophage (red: CD86 positive cell, green: CD206 positive cell, blue: DAPI to indicate the nucleus) in ADSCs treatment pancreatitis mouse pancreas (n = 6, Bar = 50 μm). The data were represented as mean ± SD, the post hoc Tukey correction was performed after ANOVA was used for the comparison of more than two groups. , .

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