Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

Figure 14

(a) XPS C_1s line of N+ PIII-treated UHMWPE without (a) and with (b) attached HRP protein and difference between these two spectra (c). (b) XPS N_1s line of N+ PIII-treated UHMWPE without (a) and with (b) attached HRP protein and difference between these two spectra (c). (c) XPS O_1s line of N+ PIII-treated UHMWPE without (a) and with (b) attached HRP protein and difference between these two spectra (c) with two fitted individual peaks. (d) XPS S_2p lines of N+ PIII-treated UHMWPE without (a) and with (b) attached HRP protein.

(a)

(b)

(c)

(d)