Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene
Figure 16
(a) Absorbance of TMB solution in dependence on the immersing time of UHMWPE surface with attached HRP. The curves of various PIII treatment time and the curve for untreated polyethylene are marked. (b) Catalytic activity of attached HRP on PIII-treated polyethylene in dependence of protein amount determined by FTIR ATR spectra. Full square is activity of HRP in solution with the concentration proportional to a complete monolayer of the protein on the surface.
