Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

<table>Stages of protein attachment: untreated polyethylene with high contact angle; PIII-treated polyethylene with high concentration of free radicals and low contact angle; aged PIII modified polyethylene with oxygen-containing groups and residual free radicals stabilised on <i>π</i>-electrons; protein is bounded to residual free radicals.</table>

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Figure 20

Figure 20: Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene 