Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

Table 1

Amount of attached HRP protein (determined from FTIR ATR using (5)) on UHMWPE treated by PIII with 20 keV energy Nitrogen ions at 10¹⁶ ions/cm² fluence.

Substrate	As attached	Washed in SDS
Untreated	0.0093±0.0014	0.0005±0.0007
PIII treated, new	0.0171±0.0001	0.0095±0.0001
PIII treated, after 2 years	0.0136±0.0004	0.0085±0.0021