Scientifica

Research Article

The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe

Table 1

Fission yeast strains used in this study.
StrainGenotype
OSP210h−, leu1-32, ura4-D18, sxa2Δ
OSP210-0h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7
OSP210-1h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Udhc1-GFP-LPI
OSP210-2h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam2-GFP-LPI
OSP210-3h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam3-GFP-LPI
OSP210-4h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Urgs1-GFP-LPI
OSP210-5h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-USPBC4.01-GFP-LPI
OSP210-6h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Uspk1-GFP-LPI
OSP210-7h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Usxa2-GFP-LPI
OSP210-8h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Udhc1-GFP-Ddhc1
OSP210-9h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam2-GFP-Dmam2
OSP210-10h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam3-GFP-Dmam3
OSP210-11h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Urgs1-GFP-Drgs1
OSP210-12h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-USPBC4.01-GFP-DSPBC4.01
OSP210-13h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Uspk1-GFP-Dspk1
OSP210-14h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Usxa2-GFP-Dsxa2
OSP210-15h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam2-GFP-DSPBC4.01
OSP210-16h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Umam3-GFP-DSPBC4.01
OSP210-17h−, leu1-32, ura4-D18, sxa2Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01
OSP220h−, leu1-32, ura4-D18, sxa2Δ, cyr1Δ
OSP220-0h−, leu1-32, ura4-D18, sxa2Δ, cyr1Δ, pSU1Z, pAL7
OSP220-2h−, leu1-32, ura4-D18, sxa2Δ, cyr1Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01
OSP220-17h−, leu1-32, ura4-D18, sxa2Δ, cyr1Δ, pSU1Z, pAL7-Umam2-GFP-LPI
OSP230h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ,
OSP230-17h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01
OSP230-17n1h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01-Pnmt1-mam2
OSP230-17u1h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01-Purg1-mam2
OSP230-17h1h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z, pAL7-Usxa2-GFP-DSPBC4.01-PhCMV-mam2
OSP230-17n2h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z-Pnmt1-mam2, pAL7-Usxa2-GFP-DSPBC4.01
OSP230-17u2h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z-Purg1-mam2, pAL7-Usxa2-GFP-DSPBC4.01
OSP230-17h2h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z-PhCMV-mam2, pAL7-Usxa2-GFP-DSPBC4.01
OSP230-2h2h−, leu1-32, ura4-D18, sxa2Δ, mam2Δ, pSU1Z-PhCMV-mam2, pAL7-Umam2-GFP-LPI