Table 2: The reporter plasmids and the GFP production of those transformants.

Plasmid name Reporter region of reporter plasmidResultant strainFluorescence intensity atSNRc
upstreamreporterdownstream0 h24 h (−)a24 h (+)b

pAL7-Udhc1-GFP-LPIdhc1 GFPLPI OSP210-11.07 (±0.12)1.02 (±0.03)1.05 (±0.04)1.03
pAL7-Umam2-GFP-LPImam2 GFPLPI OSP210-21.14 (±0.10)4.05 (±0.43)28.62 (±5.74)7.07
pAL7-Umam3-GFP-LPImam3 GFPLPI OSP210-31.05 (±0.10)1.02 (±0.10)7.66 (±2.21)7.51
pAL7-Urgs1-GFP-LPIrgs1 GFPLPI OSP210-41.52 (±0.14)1.88 (±0.23)4.12 (±0.98)2.19
pAL7-USPBC4.01-GFP-LPISPBC4.01GFPLPI OSP210-51.06 (±0.13)1.09 (±0.06)3.75 (±2.64)3.44
pAL7-Uspk1-GFP-LPIspk1 GFPLPI OSP210-61.15 (±0.08)1.58 (±0.18)3.71 (±0.74)2.35
pAL7-Usxa2-GFP-LPIsxa2 GFPLPI OSP210-71.05 (±0.09)1.05 (±0.09)9.82 (±2.09)9.35
pAL7-Udhc1-GFP-Ddhc1 dhc1 GFPdhc1 OSP210-81.07 (±0.12)1.02 (±0.03)1.06 (±0.05)1.04
pAL7-Umam2-GFP-Dmam2 mam2 GFPmam2 OSP210-91.04 (±0.13)1.15 (±0.20)6.46 (±1.06)5.62
pAL7-Umam3-GFP-Dmam3 mam3 GFPmam3 OSP210-101.05 (±0.13)0.99 (±0.11)4.02 (±1.68)4.06
pAL7-Urgs1-GFP-Drgs1 rgs1 GFPrgs1 OSP210-111.44 (±0.09)2.36 (±0.90)7.96 (±3.27)3.37
pAL7-USPBC4.01-GFP-DSPBC4.01SPBC4.01GFPSPBC4.01OSP210-121.05 (±0.08)1.03 (±0.31)7.84 (±1.85)7.61
pAL7-Uspk1-GFP-Dspk1 spk1 GFPspk1 OSP210-131.17 (±0.15)1.25 (±0.03)4.64 (±1.31)3.71
pAL7-Usxa2-GFP-Dsxa2 sxa2 GFPsxa2 OSP210-141.12 (±0.17)1.03 (±0.06)8.43 (±2.91)8.18
pAL7-Umam2-GFP-DSPBC4.01mam2 GFPSPBC4.01OSP210-150.99 (±0.02)1.21 (±0.15)6.99 (±3.19)5.78
pAL7-Umam3-GFP-DSPBC4.01mam3 GFPSPBC4.01OSP210-161.03 (±0.03)0.99 (±0.08)6.40 (±2.96)6.46
pAL7-Usxa2-GFP-DSPBC4.01sxa2 GFPSPBC4.01OSP210-170.99 (±0.04)1.06 (±0.05)15.54 (±3.09)14.66

Each value of fluorescence intensity was mean (±SD) from more than three independent experiments. a(−): incubation without P-factor. b(+): incubation with P-factor. cSNR: signal to noise ratio, SNR was obtained by dividing the fluorescence intensity at 24 h (+) by that at 24 h (−).