The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe
Table 2
The reporter plasmids and the GFP production of those transformants.
Plasmid name
Reporter region of reporter plasmid
Resultant strain
Fluorescence intensity at
SNRc
upstream
reporter
downstream
0 h
24 h (−)a
24 h (+)b
pAL7-Udhc1-GFP-LPI
dhc1
GFP
LPI
OSP210-1
1.07 (±0.12)
1.02 (±0.03)
1.05 (±0.04)
1.03
pAL7-Umam2-GFP-LPI
mam2
GFP
LPI
OSP210-2
1.14 (±0.10)
4.05 (±0.43)
28.62 (±5.74)
7.07
pAL7-Umam3-GFP-LPI
mam3
GFP
LPI
OSP210-3
1.05 (±0.10)
1.02 (±0.10)
7.66 (±2.21)
7.51
pAL7-Urgs1-GFP-LPI
rgs1
GFP
LPI
OSP210-4
1.52 (±0.14)
1.88 (±0.23)
4.12 (±0.98)
2.19
pAL7-USPBC4.01-GFP-LPI
SPBC4.01
GFP
LPI
OSP210-5
1.06 (±0.13)
1.09 (±0.06)
3.75 (±2.64)
3.44
pAL7-Uspk1-GFP-LPI
spk1
GFP
LPI
OSP210-6
1.15 (±0.08)
1.58 (±0.18)
3.71 (±0.74)
2.35
pAL7-Usxa2-GFP-LPI
sxa2
GFP
LPI
OSP210-7
1.05 (±0.09)
1.05 (±0.09)
9.82 (±2.09)
9.35
pAL7-Udhc1-GFP-Ddhc1
dhc1
GFP
dhc1
OSP210-8
1.07 (±0.12)
1.02 (±0.03)
1.06 (±0.05)
1.04
pAL7-Umam2-GFP-Dmam2
mam2
GFP
mam2
OSP210-9
1.04 (±0.13)
1.15 (±0.20)
6.46 (±1.06)
5.62
pAL7-Umam3-GFP-Dmam3
mam3
GFP
mam3
OSP210-10
1.05 (±0.13)
0.99 (±0.11)
4.02 (±1.68)
4.06
pAL7-Urgs1-GFP-Drgs1
rgs1
GFP
rgs1
OSP210-11
1.44 (±0.09)
2.36 (±0.90)
7.96 (±3.27)
3.37
pAL7-USPBC4.01-GFP-DSPBC4.01
SPBC4.01
GFP
SPBC4.01
OSP210-12
1.05 (±0.08)
1.03 (±0.31)
7.84 (±1.85)
7.61
pAL7-Uspk1-GFP-Dspk1
spk1
GFP
spk1
OSP210-13
1.17 (±0.15)
1.25 (±0.03)
4.64 (±1.31)
3.71
pAL7-Usxa2-GFP-Dsxa2
sxa2
GFP
sxa2
OSP210-14
1.12 (±0.17)
1.03 (±0.06)
8.43 (±2.91)
8.18
pAL7-Umam2-GFP-DSPBC4.01
mam2
GFP
SPBC4.01
OSP210-15
0.99 (±0.02)
1.21 (±0.15)
6.99 (±3.19)
5.78
pAL7-Umam3-GFP-DSPBC4.01
mam3
GFP
SPBC4.01
OSP210-16
1.03 (±0.03)
0.99 (±0.08)
6.40 (±2.96)
6.46
pAL7-Usxa2-GFP-DSPBC4.01
sxa2
GFP
SPBC4.01
OSP210-17
0.99 (±0.04)
1.06 (±0.05)
15.54 (±3.09)
14.66
Each value of fluorescence intensity was mean (±SD) from more than three independent experiments. a(−): incubation without P-factor. b(+): incubation with P-factor. cSNR: signal to noise ratio, SNR was obtained by dividing the fluorescence intensity at 24 h (+) by that at 24 h (−).
