SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
Figure 4
Depletion of SKIP decreases Smad3 and enhances ERK1,2 signaling by TGF-β1. (a) Smad3 and ERK1,2 phosphorylation after 60 min of TGF-β1 treatment, determined by western blot in PDV/M and AS-S cells. (b) Elk-1-dependent transcription after 24 h of TGF-β1 treatment in PDV/M and AS-S cells. (c) Transcriptional activity of ERK1,2 in PDV/M and AS-S cells determined by using SRE-luc reporter. RLU, relative luciferase units.
