Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B

Table 1

Key genetic features of three fully sequenced hPIV-4B strains.

Virus designation		SKPIV-4	68–333	04-13
Genbank accession number		EU627591	AB543337	JQ241176.1
cRNA genome length		17,361 bp	17,304 bp	17,304 bp
Leader sequenc		>55 nt?	55 nt	55 nt
First 20 nt of viral genome complimentary to terminal 20 nt?		No	Yes	Yes
Putative 3′ peptide?		—	nt 52–75	nt 52–75
ORF 1 NP		551 aa	551 aa	551 aa
		nt 155–1,810	nt 101–1,756	nt 101–1,756
CTAAGAT in 1st intergenic region		Yes	Yes	Yes
ORF 2		399 aa	399 aa	399 aa
		nt 2,096–3,293	nt 2,041–3,238	nt 2,041–3,238
		229 aa	229 aa	229 aa
		nt 2,096–2,785	nt 2,041–2,730	nt 2,041–2,730
CAGAAGTA in 2nd intergenic region		Yes	Yes	Yes
ORF 3 M		382 aa	382 aa	382 aa
		nt 3,589–4,737	nt 3,531–4,670	nt 3,531–4,670
TCTGACACACAGCT; AGAGCCA AATAC in 3rd intergenic region		TCTGACACACAACT-AAAGCCAAGCAT	Match	TCTGACACACAGCT-AGAGCCAAATAT
ORF 4 F		543 aa	543 aa	543 aa
		nt 5,232–6,863	nt 5,174–6,805	nt 5,174–6,805
CTATTAT follows poly A in 4th intergenic region		Yes	Yes	Yes
ORF 5 HN		579 aa	574 aa	574 aa
		nt 7,563–9,302	nt 7,506–9,230	nt 7,506–9,230
ORF 6 L		2,279 aa	2,279 aa	2,279 aa
		nt 10,025–16,864	nt 9,970–16,809	nt 9,970–16,809

eader sequence as defined by Komada et al. [6].