An Elegant Biosensor Molecular Beacon Probe: Challenges and Recent Solutions

Figure 5

Hairpin inversion MB probes [100]. The terminal stem-forming sequences are connected by the 3′-3′ or 5′-5′ phosphodiester linkages (left). In complex with a target, the orientation of DNA strand in stem-forming fragments is the same as in the analyte, which eliminates a possibility of hybridization.