An Elegant Biosensor Molecular Beacon Probe: Challenges and Recent Solutions

Figure 6

Using a universal MB (UMB) probe as a part of tricomponent X sensor for recognition of specific DNA or RNA sequences [98, 108]. Adaptor 𝐴 and 𝐵 strands hybridize to both analyzed sequence and a universal near-optimal MB probe reporter to form fluorescent tetrapartite complex. Triethylene glycol linkers (TEG) that connect the MB-binding arms with the analyte-binding arms are shown as dashed lines.