An Elegant Biosensor Molecular Beacon Probe: Challenges and Recent Solutions
Figure 8
Analysis of structured nucleic acids using the helper DNA strands. (a) MB-based tricomponent sensor. F strand uses long analyte-binding arm to unwind the analyte’s secondary structure. M strand binds to the opened analyte fragment only if an SNP site matches the analyte-binding arm of M strand. The two adaptor strands joint by the analyte open MB probe, thus producing high fluorescence. (b) Y-shaped junction probes. An assistant probe tightly binds and unwinds the analyte and together with MB probe forms fluorescent three-way junction complex. MB probe is partially complementary to the SNP site.
