512840.fig.0012
Figure 12: Engineering mesophilic-like activity in mutants of a psychrophilic α-amylase. This plot of the kinetic parameters for the stabilized mutants (filled symbols) shows that the general trend is to decrease the activity and to increase the affinity for the substrate of the wild-type psychrophilic enzyme (open symbol). The most stable mutant bearing six additional interactions (arrow) displays kinetic parameters nearly identical to those of the mesophilic homologue (open symbol). Adapted from [189, 273].