Global Molecular Epidemiology of Cryptococcus neoformans and Cryptococcus gattii: An Atlas of the Molecular Types

Table 1

Genotype nomenclature adopted by the main molecular typing techniques and correspondence to standard nomenclature.

Species/variety	Standard; nomenclature	PCR-fingerprinting M13 [15]	PCR-fingerprinting (GACA)4[155]	AFLP; [11]	MLST; [14, 53]	Reference strains
C. neoformans; var. grubii	VNI	VNI	VN6	AFLP1	VNI	ATCC MYA-4564 [15]
	VNII	VNII	VN6	AFLP1A or AFLP1B	VNII	ATCC MYA-4565 [15]
	VNB	VNI or VNII	VN6	AFLP1A or AFLP1B	VNB	bt1, bt131, bt100 [53]
C. neoformans; var. neoformans	VNIV	VNIV	VN1	AFLP2	—	ATCC MYA-4567 [15]
Inter-varietal; AD hybrids	VNIII	VNIII	VN3 or VN4	AFLP3	—	ATCC 32045 [168]
C. gattii	VGI	VGI	—	AFLP4	VGI	ATCC MYA-4560 [64]
	VGII	VGII	—	AFLP6	VGII	ATCC MYA-4561 [64]
	VGIII	VGIII	—	AFLP5	VGIII	ATCC MYA-4562 [64]
	VGIV	VGIV	—	AFLP7	VGIV	ATCC MYA-4563 [64]
Inter-species; hybrids	VNI/VGI	VNI/VGI	—	AFLP9	—	CBS 10496 [122]
	VNIV/VGI	VNIV/VGI	—	AFLP8	—	CBS 10488 [122]
	VNI/VGII	VNI/VGII	—	—	—	WM 05-272 [29]

ATCC: American Type Culture Collection (http://www.atcc.org/); CBS: Centraalbureau voor Schimmelcultures (http://www.cbs.knaw.nl/); WM: Westmead Millennium Institute, Sydney, Australia; bt: isolate from Botswana.