Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis
Figure 4
2D gel analysis. (a) Histone proteins were enriched from Arabidopsis young seedling lysate and stained with Coomassie Blue. The same amount of total and histone-enriched proteins was separated by 15% SDS-PAGE and the western blot results showed that histone proteins were enriched from Arabidopsis young seedling lysate. W: the total isolated proteins. H: the histone-enriched proteins. Ct: commercial calf thymus histones (Sigma). (b) The histone-enriched proteins were analyzed by 20% SDS-PAGE with pH gradient from 9 to 12. The biotin signals that concentrate at pH 9 versus the core-histones signals that distribute between pH 10 to 11. Experiments were conducted in triplicate.
