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Scientifica
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2014
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Article
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Tab 12
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Research Article
Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
Table 12
Advantages of XF H and E method.
Routine H and E
1.5% DWS
95% DLW
Cost
High
Low
Low
Time
50–55 min
25–30 min
54 min
Toxicity
Present
Absent
Absent
Biohazardous
Yes
No
No
Inflammability of chemicals used
Present
Absent
Absent
Staining protocol
Lengthy
Simplified
Simplified
Handling
Toxic if not properly handled
Easy
Easy
Quality of staining
Good
Good
Good
Disposal of chemicals
Difficult
Easy
Easy
Preparation
Synthetic
Synthetic
Naturally available from plants