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Scientifica
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2014
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Article
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Tab 2
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Research Article
Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
Table 2
Routine H and E staining procedure.
Deparaffinization and Rehydration
Xylene
25 min
90% alcohol
1 min
70% alcohol
1 min
Water wash
1 min
Nuclear staining
Harris hematoxylin
8 min
Tap water wash
2 min
Differentiation
Differentiation in 1% acid alcohol
1 dip
Bluing
1% lithium carbonate
1 min
Water wash
10 min
Cytoplasmic staining
1% eosin
1 min
Dehydration
70% alcohol
30 sec
90% alcohol
30 sec
Xylene
1 min
Approximate time required
50 to 55 min