Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study

Table 3

Xylene and alcohol free H and E staining procedure where diluted dish wash was used as deparaffinizing agent.

Deparaffinization	Diluted dish washing soap 1.5%-I	At 90°C	1 min
	Diluted dish washing soap 1.5%-II	At 90°C	1 min
	Distilled water-I	At 90°C	30 sec
	Distilled water-II	At 90°C	30 sec
	Wash slides in distilled water	At 45°C	30 sec
	Wash slides in distilled water	At room temperature	30 sec
Nuclear staining	Harris hematoxylin	At room temperature	8 min
	Tap water wash		2 min
Differentiation	Differentiation in 1% acid alcohol	At room temperature	1 dip
Bluing	Tap water wash		10 min
Cytoplasmic staining	1% eosin	At room temperature	1 min
	Tap water wash		1 min
	Wash slides in distilled water
Dehydration	Over drying the sections	At 60°C	10 min
	Approximate time required		25 to 30 min