Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study

Table 4

Xylene and alcohol free H and E staining procedure using 95% lemon water as deparaffinizing agent.

Deparaffinization	Diluted lemon water 95%-I	At 94°C	5 min
	Diluted lemon water 95%-II	At 94°C	5 min
	Distilled water-I	At 94°C	5 min
	Distilled water-II	At 94°C	5 min
	Wash slides in distilled water	At 45°C	30 sec
	Wash slides in distilled water	At room temperature	30 sec
Neutralizing the effect of acidity in lemon water	Lithium carbonate		15 min
	Tap water wash		5 min
Nuclear staining	Harris hematoxylin	At room temperature	2 min
	Tap water wash		2 min
Differentiation	Differentiation in 1% acid alcohol	At room temperature	1 dip
Bluing	Tap water wash		10 min
Cytoplasmic staining	1% eosin	At room temperature	1 dip
	Tap water wash		1 min
	Wash slides in distilled water
Dehydration	Over drying the sections	At 60°C	5 min
	Approximate time required		54 min