Common ice crystal morphology changes based antifreeze activity present. (a) Prior to ice crystal shaping, ice crystals are a spherical shape. (b) When there is a lack of ice crystal shaping, the crystal grows and remains circular (control buffer only). (c) Low level of antifreeze activity is represented by a hexagonal ice crystals. (d) Ice crystals seen with moderate level of antifreeze activity are usually observed as hexagonal bipyramids.
