Chloroplast DNA Variations in Wild Brassicas and Their Implication in Breeding and Population Genetics Studies

Table 3

Polymorphic fragments obtained with various primer pair-restriction enzyme combinations to reveal intraspecific variations in wild brassicas.

Species	Accession	Polymorphic fragments^a (bp) in various primer pair-restriction enzyme combinations
		CD-(HinfI)1	CD-(HinfI)2	CD-(TaqI)1
B. elongata	EC662178	330	0	940
	EC662179	330	0	940
	EC662180	310	150	580 + 210 + 150
	EC662182	330	0	940
B. insularis	EC675815	110	—	—
	EC675816	110	—	—
	EC675817	0	—	—
D. catholica	EC662194	330	—	550
	EC662195	350	—	570
		DT-(HinfI)1	DT-(HinfI)2	DT-(TaqI)1	DT-(TaqI)2
B. cretica	EC662183	500	—	310	—
	EC662184	475	—	0	—
B. elongata	EC662178	0	—	220	—
	EC662179	0	—	210	—
	EC662180	210	—	220	—
	EC662182	0	—	220	—
B. insularis	EC675815	470	140	230	140
	EC675816	470	140	230	140
	EC675817	480	0	320	0
B. maurorum	EC662185	170	—	230	—
	EC662186	175	—	220	—
	EC662187	170	—	230	—
		HK-(HinfI)1
B. barrelieri	EC662175	0
	EC662176	210
	EC662177	210
D. catholica	EC662194	190
	EC662195	170
		K1K2-(HinfI)1	K1K2-(HinfI)2	K1K2-(TaqI)1	K1K2-(TaqI)2	K1K2-(TaqI)3	K1K2-(TaqI)4
B. cretica	EC662183	370	200	330	—	—
	EC662184	380	210	240	—	—
B. elongata	EC662178	—	—	970	520	410	340
	EC662179	—	—	600 + 370	510	410	230
	EC662180	—	—	600 + 370	510	400	340
	EC662182	—	—	600 + 370	520	410	230
B. insularis	EC675815	200	—	320 + 300	510	290
	EC675816	200	—	320 + 300	510	290
	EC675817	205	—	620	520	250
D. assurgens	EC662192	220	—	—	—	—
	EC662193	230	—	—	—	—
D. virgata	EC662198	230	—	210	—	—
	EC662199	370	—	240	—	—
		TF-(HinfI)1	TF-(HinfI)2	TF-(Taq)1
B. cretica	EC662183	510	360	—
	EC662184	600	350	—
B. insularis	EC675815	610	350	200
	EC675816	610	350	200
	EC675817	510	360	210
		VL-(HinfI)1	VL-(TaqI)1
B. insularis	EC675815	230	420
	EC675816	230	420
	EC675817	240	510

The numbers (1 to 4) shown besides each primer pair-restriction enzyme combination indicate the order of polymorphic fragments (highest to lowest size).