Chloroplast DNA Variations in Wild Brassicas and Their Implication in Breeding and Population Genetics Studies
Table 3
Polymorphic fragments obtained with various primer pair-restriction enzyme combinations to reveal intraspecific variations in wild brassicas.
Species
Accession
Polymorphic fragmentsa (bp) in various primer pair-restriction enzyme combinations
CD-(HinfI)1
CD-(HinfI)2
CD-(TaqI)1
B. elongata
EC662178
330
0
940
EC662179
330
0
940
EC662180
310
150
580 + 210 + 150
EC662182
330
0
940
B. insularis
EC675815
110
—
—
EC675816
110
—
—
EC675817
0
—
—
D. catholica
EC662194
330
—
550
EC662195
350
—
570
DT-(HinfI)1
DT-(HinfI)2
DT-(TaqI)1
DT-(TaqI)2
B. cretica
EC662183
500
—
310
—
EC662184
475
—
0
—
B. elongata
EC662178
0
—
220
—
EC662179
0
—
210
—
EC662180
210
—
220
—
EC662182
0
—
220
—
B. insularis
EC675815
470
140
230
140
EC675816
470
140
230
140
EC675817
480
0
320
0
B. maurorum
EC662185
170
—
230
—
EC662186
175
—
220
—
EC662187
170
—
230
—
HK-(HinfI)1
B. barrelieri
EC662175
0
EC662176
210
EC662177
210
D. catholica
EC662194
190
EC662195
170
K1K2-(HinfI)1
K1K2-(HinfI)2
K1K2-(TaqI)1
K1K2-(TaqI)2
K1K2-(TaqI)3
K1K2-(TaqI)4
B. cretica
EC662183
370
200
330
—
—
EC662184
380
210
240
—
—
B. elongata
EC662178
—
—
970
520
410
340
EC662179
—
—
600 + 370
510
410
230
EC662180
—
—
600 + 370
510
400
340
EC662182
—
—
600 + 370
520
410
230
B. insularis
EC675815
200
—
320 + 300
510
290
EC675816
200
—
320 + 300
510
290
EC675817
205
—
620
520
250
D. assurgens
EC662192
220
—
—
—
—
EC662193
230
—
—
—
—
D. virgata
EC662198
230
—
210
—
—
EC662199
370
—
240
—
—
TF-(HinfI)1
TF-(HinfI)2
TF-(Taq)1
B. cretica
EC662183
510
360
—
EC662184
600
350
—
B. insularis
EC675815
610
350
200
EC675816
610
350
200
EC675817
510
360
210
VL-(HinfI)1
VL-(TaqI)1
B. insularis
EC675815
230
420
EC675816
230
420
EC675817
240
510
The numbers (1 to 4) shown besides each primer pair-restriction enzyme combination indicate the order of polymorphic fragments (highest to lowest size).