Short Wavelength Cone Opsin Is Not Expressed in the Retina of Arboreal African Pangolin (Manis tricuspis)

Figure 1

(a) Strong immunoreactivity with PNA cone marker in ONL and cone terminals across all vertical sections of pangolin retina. (b) Strong expression of L-cone opsin across all vertical sections of pangolin retina. (c) S-cone opsin absent across all vertical sections of pangolin retina. Blue arrow indicates cone photoreceptors, PNA is peanut agglutinin, INL is inner nuclear layer, OPL is outer plexiform layer, ONL is outer nuclear layer, L— is long wavelength, and S- is short wavelength. Scale bar 20 m. (d) Triple labeling showed strong immunoreactivity with PNA cone marker (orange) in the ONL and in the terminals in the INL, across all vertical sections of pangolin retina. L-cones opsins are strongly expressed in the ONL (green). Short wavelength cones are not expressed across all vertical sections of the retina. Yellow arrow indicates cone photoreceptor. D GCL iOPL is outer plexiform layer, IPL is inner plexiform layer, ONL is outer nuclear layer, and INL is inner nuclear layer. Scale bar is 20 m.

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