A Comprehensive Exploration of the Fatty Acids Profile, Cholesterol, and Tocopherols Levels in Liver from Laying Hens Fed Diets Containing Nonindustrial Hemp Seed

Table 1

Formulation, proximate chemical composition, and nutrient profile of diets containing hempseed (HS) compared with the control.

Item	Diet
	Control	HS-10	HS-20	HS-30
Ingredients (g·kg^−1)
Corn	588.29	462.14	380.01	212.94
Hemp seed	0	100	200	300
Soybean meal	255.85	104.59	95.63	110.94
Sunflower meal	16.85	130	130	130
Soybean oil	20.28	10	0	0
Calcium	86.09	73.79	89.34	89.27
DDGS	8.96	70	70	35.72
Di calcium phosphate	13.68	37.26	11.62	20
Sodium sulfate	0	2.37	3	2
Sodium chloride	1.83	1.12	2	2
DL-methionine	2.73	2.15	2.17	2
L-Lysine HCl	0.1	1.46	9.99	15
Vitamin premix	5	5	6.11	80
Mineral premix	0.4	0.12	0.13	0.13
Composition (calculated unless noted)
AME (kcal/kg)	2990	3000	3000	3000
Crude fat (%)	5.06	7.14	8.48	11.10
Crude protein (%)	17.8	17.8	18	18
Calcium (g/kg)	39	40	40	40
Phosphorus available (%)	0.44	0.85	2.78	2.78
Sodium (g/kg)	1.1	2	2	2
α-Linolenic acid (%)	2.49	7.45	12.09	17.37
Lysine (g/kg)	9.35	8.64	8.71	8.74
Methionine (g/kg)	5.58	5.59	5.82	6.14
Threonine (g/kg)	6.90	6.63	6.94	6.91

DDGS: distiller’s dried soluble grains; AME available metabolizable energy. Providing per g of premix, 11,00 IU vitamin A; 3,000 IU vitamin D3; 0.3 mg vitamin K3; 0.22 mg vitamin B1; 0.65 mg vitamin B2; 0.45 mg vitamin B6; 2 μg vitamin B12; 1 mg pantothenic acid; 2.5 mg niacin; 0.4 mg folic acid; 15 IU vitamin E; 23 mg choline chloride; 0.30 mg sodium; 66 mg manganese; 80 mg iron; 70 mg zinc; 10 mg copper; 0.4 mg iodine; 36 mg magnesium; 0.10 mg selenium.