764830.fig.002a
(a)
764830.fig.002b
(b)
764830.fig.002c
(c)
764830.fig.002d
(d)
Figure 2: Pimonidazole stain identifies penumbra. (a) Intense staining is present in penumbra (arrow) adjacent to infarct (stars), 0.5× magnification. (b) Closeup of area within the box demonstrates intense staining adjacent to infarct, 4× magnification. (c) Diffuse weak staining (arrows) indicates mild hypoxia associated with euthanasia. Infarct does not stain (star.), 0.5× magnification. Negative control (d).