Stroke Research and Treatment

Research Article

Functional Inflammatory Genotypes in Ischemic Stroke: Could We Use Them to Predict Age of Onset and Long-Term Outcome?

Table 1

Allele-specific primers and real-time PCR conditions.
Allele-specific primersReal-time PCR conditions
IL4-589C>TSegment 1-Amplification (40 cycles)
(i) Denaturation at 95°C for 3 0
(ii) Annealing at 56°C for 3 0
(iii) Extension at 72°C for 2 0
Segment 2-Qualitative data collection (115 cycles):
70–95°C for 1 8
(Specifically for TNF-α-308G>A: annealing at 61°C for 2 5 )
 FRW C primer 
 FRW T primer		5′-GCGGCGCGGCCCACTAAACTTGGGAGAACATTGA C-3′
5′-AGTAAACTTGGGAGAACATTGTT-3′
 REV primer5′-GGCAGAATAACAGGCAGAC-3′
IL10-1082G>A
 FRW G primer 
 FRW A primer		5′-GCGGCGCGGCGG-TTAGTAAGGCTTCTTTGGGT G -3′
5′-TACTACTAAGGCTTCTTTGGGAA -3′
 REV primer5′-CTGGATAGGAGGTCCCTTAC-3′
TNF- 𝛼 -308G>A
 FRW G primer*5′-GCGGCGCGGCGG-CTGGCTGAACCCCGTCT C-3′
 FRW A primer*5′-AGGCTGAACCCCGTGCT-3′
 REV primer*5′-AAGGAAACAGACCACAGACCTG-3′
IL6-174G>C
 FRW G primer*5′-ATTGTGACGTCCTTTAGCATC-3′
 FRW C primer*5′-GCGGGCGGGCCG-ATTGTGACGTCCTTTAGGTTG-3′
 REV primer*5′-ATGACGACCTAAGCTGCAC-3′
IL12B 1188A>C
 FRW A primer*5′-TTTCAATGAGCATTTAGCAACT-3′
 FRW C primer*5′-GCGGCGCGGCGG-TACAATGAGCATTTAGCATG G-3′
 REV primer*5′-TAGGATCACAATGATATCTTTGC-3′
*Primer designed on the complementary sequence. FRW: forward, REV: reverse.
Underlined are sites of introduced mismatches. Bold are the polymorphic alleles. Italic is the intoduced GC-tail.