Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model
Figure 3
(a) STAT3 phosphorylation in the cerebral cortex of the ischemic brain. Photomicrograph illustrates the presence of phosphorylated STAT3 in the vessels of the ischemic brain at 2 hours after ischemic insult. Positive immunoreactivity is not observed in nonischemic brain (sham), and hypothermia (33) reduced the number of phosphorylated STAT3 positive cell compared with normothermic group (37). Scale bar: 100 μm. (b) Western blot analysis shows that ischemia-induced STAT3 phosphorylation at 2 and 6 hours after MCAO onset in normothermic brain (37, ) is reduced in hypothermia group (33, ). * versus normothermia. (c) Western blot analysis demonstrates that ICAM-1 induction at 24 hours after MCAO is reduced by STAT3 inhibitor treatment (JSI, ). * versus sham; # versus ischemia without JSI-124.
