Lactoferrin Augmentation of the BCG Vaccine Leads to Increased Pulmonary Integrity
Figure 3
Protective histopathology following infectious challenge in lactoferrin adjuvant immunized mice. C57BL/6 mice were immunized as detailed in Figure 2 with BCG or BCG/bovine lactoferrin (10 or 100 μg/mouse) and aerosol challenged 12 weeks after final boost. At day 65 postchallenge, lungs were collected, formalin fixed, and stained with hematoxylin and eosin (H&E). Comparison is made to nonimmunized, infected controls. A mixed presence of lymphocytes and activated macrophages indicative of a protective response is identified in the BCG alone vaccinated group. The addition of lactoferrin at either concentration resulted in a confined granulomatous response with focal lymphocytic accumulation and limited to no aggravated macrophage or polymorphonuclear insult. Images were visualized at 100x magnification.
