Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR

Table 1

Real-time PCR results showing drop in 𝑇_𝑚. (wild/mutant rpob gene with different 𝑇_𝑚, Bin A: Wild, Bin B: Mutant).

No.	Colour	Name	Genotype	Peak 1	Peak 2
1		MTB h37 Rv WILD	Wild	74.7 (Bin A)
2		001 DEL/07	Wild	74.0 (Bin A)
3		002 DEL/07	Mutant	70.3 (Bin B)	75.5
4		003 DEL/07	Mutant	70.3 (Bin B)	75.3
5		004 DEL/07	Wild	74.5 (Bin A)
6		005 DEL/07	Wild	74.3 (Bin A)
7		006 DEL/07	Wild	74.5 (Bin A)
8		007 DEL/07	Mutant	70.5 (Bin B)
9		008 DEL/07	Mutant	70.5 (Bin B)	75.2
10		009 DEL/07	Mutant	70.5 (Bin B)	75.8
11		0010 DEL/07	Mutant	70.7 (Bin B)	75.5
12		0011 DEL/07	Mutant	70.5 (Bin B)
13		0012 DEL/07	Wild	74.5 (Bin A)
14		0013 DEL/07	Wild	74.5 (Bin A)
15		0014 DEL/07	Wild	74.5 (Bin A)
16		0015 DEL/07	Mutant	70.5 (Bin B)	75.5
17		0016 DEL/07	Mutant	70.5 (Bin B)
18		0017 DEL/07	Wild	74.2 (Bin A)
19		0018 DEL/07	Mutant	70.5 (Bin B)	75.0
20		0019 DEL/07	Wild	74.5 (Bin A)
21		0020 DEL/07	Mutant	70.5 (Bin B)	75.5
22		0021 DEL/07	Mutant	70.3 (Bin B)	76.0
23		0022 DEL/07	Wild	74.5 (Bin A)
24		0023 DEL/07	Mutant	70.5 (Bin B)	75.5
25		0024 DEL/07	Mutant	70.5 (Bin B)	75.8
26		0025 DEL/07	Wild	74.7 (Bin A)
27		0026 DEL/07	Mutant	70.2 (Bin B)
28		0027 DEL/07	Wild	74.8 (Bin A)
29		0028 DEL/07	Mutant	70.3 (Bin B)	75.5
30		0029 DEL/07	Mutant	70.5 (Bin B)	75.5
31		0030 DEL/07	Wild	74.5(Bin A)
32		0031 DEL/07	Mtb neg sample
33		Nc

This report generated by Rotor-Gene 6000 Series Software 1.7 (Build 87) Copyright 2000–2006 Corbett Research, a Division of Corbett Life Science. All rights reserved. ISO 9001 : 2000 (Reg. no. QEC21313).