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TheScientificWorldJOURNAL
Volume 8 (2008), Pages 1269-1279
http://dx.doi.org/10.1100/tsw.2008.163
Research Article

Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells

1Department of Orthopedics, University Hospital of Marburg, D-35043 Marburg, Baldingerstraße, Germany
2Department of Chemistry, University of Marburg, D-35032 Marburg, Hans-Meerwein-Straße, Germany
3Department of Orthopedics, Sankt-Elisabeth-Hospital Gütersloh, D-33332 Gütersloh, Kattenstroth 103, Germany

Received 5 October 2008; Revised 9 December 2008; Accepted 17 December 2008

Academic Editor: Martin Goette

Copyright © 2008 Markus D. Schofer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or BMP-2–incorporated PLLA were seeded with human mesenchymal stem cells and cultivated over a period of 22 days under growth and osteoinductive conditions, and analyzed during the course of culture, with respect to gene expression of alkaline phosphatase (ALP), osteocalcin (OC), and collagen I (COL-I). Furthermore, COL-I and OC deposition, as well as cell densities and proliferation, were analyzed using fluorescence microscopy. Although the presence of nanofibers diminished the dexamethasone-induced proliferation, there were no differences in cell densities or deposition of either COL-I or OC after 22 days of culture. The gene expression of ALP, OC, and COL-I decreased in the initial phase of cell cultivation on PLLA nanofibers as compared to cover slip control, but normalized during the course of cultivation. The initial down-regulation was not observed when BMP-2 was directly incorporated into PLLA nanofibers by electrospinning, indicating that growth factors like BMP-2 might survive the spinning process in a bioactive form.