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TheScientificWorldJOURNAL
Volume 10 (2010), Pages 393-401
http://dx.doi.org/10.1100/tsw.2010.37
Research Article

Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium

1Postgraduate Medical School – Oncology, University of Surrey, Manor Park, Guildford, UK
2Oncology Department, St George's University of London, United Kingdom

Received 13 November 2009; Revised 10 February 2010; Accepted 13 February 2010

Academic Editor: Ernst Wagner

Copyright © 2010 Agnieszka Michael et al.

Abstract

Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-α, IL-12, IL-1β; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection.