Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis

Figure 3

Active and inactive osteoblast progenitor markers and housekeeping gene in suspension and adherent mononucleated cells. (A) Cbfa-1 in undifferentiated mononucleated cells. (B) Cbfa-1 in mononucleated cells after 3 days in osteoblast differentiation medium. Gapdh is represented in suspension and adherent undifferentiated mononucleated cells (Left) and mononucleated cells after 3 days in osteoblast differentiation medium (Right). Cbfa-1 was not active in mononucleated cells but was active after 3 days in osteoblast differentiation medium. The positive control, Gapdh, was active before and after differentiation of mononucleated cells into osteoblasts.