Proinflammatory Stimuli Enhance Phagocytosis of Apoptotic Cells by Neutrophil Granulocytes
Figure 1
Phagocytosis of apoptotic neutrophils by purified human neutrophil granulocytes. 1 × 105 PKH67 stained viable neutrophils were preincubated with the indicated stimuli for 30 min and, subsequently, 4 × 105 PKH26 stained apoptotic neutrophils were added. Phagocytosis was assessed by flow cytometry after 90 min. (a) Effect of TLR-ligands on the phagocytosis rate. (b) Effect of various cytokines on the phagocytosis rate. Data show mean + SD from 3 independent experiments, *:. (c)–(f) Representative dot-plots for the quantitative assessment of ingestion of apoptotic cells by purified neutrophils by using flow cytometry. (c) FSC/SSC gating of neutrophils. (d) Negative control: PKH67-labeled (green) fresh neutrophils without apoptotic cells. (e) PKH67-labeled (green) fresh neutrophils after coincubation with PKH26-labeled (red) apoptotic neutrophils in medium alone. (f) Pam3CSK4-treated PKH67-labeled (green) fresh neutrophils after coincubation with PKH26-labeled (red) apoptotic neutrophils.
