The Scientific World Journal

Clinical Study

HIV-1 Replication in HIV-Infected Individuals Is Significantly Reduced When Peripheral Blood Mononuclear Cells Are Superinfected with HSV-1

Table 1

Characteristics of PBMCs from HIV-infected individuals and healthy donors.
 ExperimentMean ± SD 
HIV-infected individualsHealthy donors 𝑃   value
𝑁 = 2 5 𝑁 = 1 5
Activated T lymphocytes 
 percentage of CD3+ and CD38+ 4 6 . 5 1 ± 1 7 . 5 4 2 7 . 5 4 ± 1 4 . 1 2 0.001
HVEM expressing T lymphocytes   
  percentage of CD3+HVEM+ 0 . 5 0 ± 0 . 5 3 0 . 5 7 ± 0 . 3 4 0.282
  percentage of CD38+HVEM+ 0 . 7 9 ± 0 . 9 2 0 . 8 0 ± 0 . 9 9 0.890
 MFI of HVEM 3 . 7 5 ± 1 . 5 7 4 . 9 2 ± 2 . 1 1 0.142
HSV-1 infected T cells  
  percentage of CD3 + HSV-1+ 7 9 . 2 5 ± 1 4 . 6 3 8 0 . 7 6 ± 7 . 1 3 0.922
  percentage of CD4+HSV-1+/Total CD4+ 9 7 . 6 0 ± 2 . 2 0 9 2 . 9 3 ± 3 . 9 0 <0.001
  percentage of CD8+HSV-1+/Total CD8+ 9 7 . 7 9 ± 3 . 6 2 9 1 . 8 9 ± 9 . 3 3 0.001
HSV-1 titers ( × 1 0 3 PFU/mL) 
 Lysate supernatant (intracellular) 3 . 5 4 ± 1 . 2 1 2 . 1 7 ± 0 . 9 0 0.001
 Culture supernatant (extracellular) 4 3 . 8 0 ± 1 1 . 3 0 3 2 . 0 0 ± 8 . 1 9 0.002
 Total amount 4 7 . 3 4 ± 1 1 . 1 4 3 4 . 1 7 ± 8 . 4 8 0.001
HIV titers from HIV-infected individuals (log10 copies/mL) 
  With HSV-1 infection 3 . 5 4 ± 1 . 0 5  <0.001
  Mock infection 3 . 8 5 ± 1 . 0 9