Mitochondrial Electron Transport Chain in Heavy Metal-Induced Neurotoxicity: Effects of Cadmium, Mercury, and Copper

Table 5

Action of different antioxidants and MPT pore inhibitors on viability of PC12 cells assessed by LDH release after different time of incubation with different concentration of Cd²⁺.

Treatment	Control cells (3, 5, 24 h)	500 μM Cd (3 h)	100 μM Cd (5 h)	100 μM Cd (24 h)
Control	100	55±3 *	74±4 *	61±5 *
Ruthenium Red (10 μM)	99±3	71±6 **	92±3 **	60±4
BKA, 5 μM	100±2	70±5 **	82±6	58±2
CsA, 1 μM	101±2	76±4 **	72±6	59±18
NAC (10 mM, 2 h)	104±4	94±2 **	96±2 **	92±5 **
Vit E, 500 μM	103±1	65±6 **	86±7 **	83±2 **
Mannitol (50 mM)	100±1		92±6 **
BHT, 150 μM	99±2		93±1 **	82±1 **
TEMPO (1 mM)	97±3		87±8 **

The cell viability is expressed in % to untreated control. Mean ± SE; 𝑛=3–11 independent experiments; *𝑃<0.05 compared to untreated control; **𝑃<0.05 compared to Cd(II)-treated control.