Plasmalogens Inhibit APP Processing by Directly Affecting γ-Secretase Activity in Alzheimer’s Disease
Figure 2
Protein level of the secretases involved in APP processing. (a) SH-SY5Y cells were incubated with PC-PL 18 : 1 and the corresponding phospholipid PC 18 : 1. Cell lysates were prepared, subjected to gel electrophoresis and Western blot (WB) analysis. Protein level of ADAM17, PS1, and BACE1 were detected with antibodies ab39162, sc-7860 and B0806, respectively. (b) Effect of PC-PL 20 : 4 on protein level of ADAM17, PS1, and BACE1 compared to the corresponding phospholipid PC 20 : 4. Detection was performed as described for (a) All quantified data represent an average of at least three independent experiments. Error bars represent standard deviation of the mean. Asterisks show the statistical significance (*; ** and ***, n.s. = not significant). (a, b) Representative WBs of protein determination and representative agarose gels of RT-PCR analyis are shown.
