The Scientific World Journal

Research Article

Plasmalogens Inhibit APP Processing by Directly Affecting γ-Secretase Activity in Alzheimer’s Disease

Table 3

RT-PCR analysis of α-secretase ADAM17, the γ-secretase components and β-secretase BACE1 in presence of PC-PL 18 : 1 and PC-PL 20 : 4 compared to the corresponding phospholipids PC 18 : 1 and PC 20 : 4 as control. The abbreviations set are ADAM17 = α-secretase, PSEN1 = presenilin1, PSEN2 = presenilin2, APH1a = anterior pharynx-defective 1a, APH1b = anterior pharynx-defective 1b, NCSTN = nicastrin, PSENEN2 = presenilin enhancer 2, and SEM = standard deviation of the mean. Statistical significance was determined by two-tailed Student’s t-test.


		Gene	% (compared to control)	SEM	t-test

PC-PL 18 : 0/18 : 1	α-secretase	ADAM17	110,65	17,775	0,5658
	γ-secretase	PSEN1	96,32	11,721	0,7574
		PSEN2	103,59	13,585	0,7981
		APH1a	87,01	23,043	0,5884
		APH1b	106,43	18,578	0,7380
		NCSTN	87,42	20,414	0,5548
		PSENEN2	108,75	14,912	0,5734
	β-secretase	BACE1	104,24	22,534	0,8554

PC-PL 18 : 0/20 : 4	α-secretase	ADAM17	90,44	4,919	0,0878
	γ-secretase	PSEN1	94,97	9,865	0,6162
		PSEN2	110,28	10,516	0,3568
		APH1a	86,45	8,927	0,1676
		APH1b	92,58	12,544	0,5707
		NCSTN	85,93	8,304	0,1287
		PSENEN2	98,99	7,433	0,8949
	β-secretase	BACE1	92,90	9,413	0,4607