Simple Reversed-Phase HPLC Method with Spectrophotometric Detection for Measuring Acetaminophen-Protein Adducts in Rat Liver Samples
Figure 2
Chromatograms of (a) a liver sample from a rat treated only with physiological saline; (b) a rat liver extract spiked with APAP-Cys (2.5 μg/100 μL) and PABA, the internal standard; (c) a liver extract from a rat treated with APAP (800 mg/kg i.p.); (d) a liver extract from a rat treated with APAP (800 mg/kg i.p.) and spiked ex vivo with authentic APAP-Cys (2.5 μg/100 μL of liver extract). Key: 1, PABA, the internal standard; 2, APAP-Cys adduct.
