Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk
Figure 6
Optimization of assay conditions with RNA aptamer. (a) Influence of the addition of Na+ ion on assay buffer; () 10 mM phosphate buffer and (∘) PBS buffer (150 mM NaCl). (b) Effect of the addition of Mg2+ ion on assay buffer. The concentration of MgCl2 was () no addition, (∘) 2.5 mM, and (▾) 5 mM. (c) Optimization of temperature on the ELAA performance: () room temperature and (∘) 4°C.
