Research Article
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk
Table 3
Analytical data for TC aptamer assay (ELAA) compared with TC antibody assay (ELISA).
| | Method | Matrix | LOD | Dynamic range | Linearity, |
| | ELAA (DNA) | Buffer | 3.27 × 10−8 M | 1.99 × 10−7 M~1.00 × 10−4 M | 0.9934 | | Milk | 9.52 × 10−8 M | 3.16 × 10−7 M~3.16 × 10−4 M | 0.9881 |
| | ELAA (RNA) | Buffer | 2.10 × 10−8 M | 3.16 × 10−8 M~3.16 × 10−4 M | 0.9850 | | Milk | 3.51 × 10−8 M | 1.0 × 10−7 M~1.0 × 10−4 M | 0.9823 |
| | ELISA | Buffer [21] | 1.74 × 10−10 M | 1.0 × 10−9 M~1.0 × 10−6 M | 0.9901 | | Milk [21] | 1.0 × 10−10 M | 3.16 × 10−10 M~3.16 × 10−7 M | 0.9862 | | Honey [22] | 3.98 × 10−10 M | 3.16 × 10−9 M~3.16 × 10−7 M | 0.9800 |
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