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The Scientific World Journal
Volume 2012 (2012), Article ID 254929, 8 pages
http://dx.doi.org/10.1100/2012/254929
Research Article

Microbial Transformations of 7-Hydroxyflavanone

Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland

Received 11 October 2011; Accepted 22 December 2011

Academic Editor: Gabriella Marucci

Copyright © 2012 Edyta Kostrzewa-Susłow and Tomasz Janeczko. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Microbial transformations of racemic 7-hydroxyflavanone using strains of genus Aspergillus (A. niger KB, A. niger 13/5, A. ochraceus 456) and the species Penicillium chermesinum 113 were studied. The products of O-methylation, O-methylation along with hydroxylation at C-3′ and C-4′, reduction of the carbonyl group, reduction of the carbonyl group along with hydroxylation at C-5, and dehydrogenation of C-2 and C-3 were obtained. Most of the products (with the exception of the O-methylation one) have stronger antioxidant properties than the initial substrate.

1. Introduction

Flavonoids comprise a large group of secondary metabolites derived from phenylalanine. They are commonly found in plants, where their biogenesis takes place under normal physiological conditions (e.g., synthesis of pigments in flowers), but also may be a part of environmental stress response (e.g., synthesis of phytoalexins) [13]. Although the presence of natural flavonoids in animals and people has not been confirmed so far, their multidirectional influence on many important processes in these organisms was proved and reported by several research institutes all over the world [47]. Among many review articles about flavonoids, the following two: “The Flavonoids” [8] and “The Handbook of Natural Flavonoids” [1], are particularly rich in information. They present a couple of thousands of known compounds of this group, including their structures, separation methods, and identification details. On the basis of many cited papers the authors describe chemical and biological properties of flavonoids, including their influence on capillary vessels structure, on functioning of heart, liver, and kidneys. They also report diuretic, antibacterial, antiviral, immunomodulatory, anticancer, hypoglycemic, and antioxidant properties of flavonoids.

Practical application of flavonoids in pharmacy or in medicine is often considerably limited due to their low solubility, inefficient transport across biological membranes or low stability. Biotransformation of flavonoid compounds may be a natural method of modification of their structures, aiming at greater structural diversity and better bioaccessibility. It also gives a possibility to trace metabolic transformation of flavonoids [912].

Enzymatic systems of microorganisms are capable of different reactions, including hydrolysis, carbonyl group reduction, hydroxylation, O-methylation, oxidation, isomerization, and also formation or breakage of C–C and C–heteroatom bonds [13, 14].

Ibrahim and Abul-Hajj described biotransformation of monohydroxyflavones with substituents in the A-ring using the strain of Streptomyces fulvissimus (NRRL 1453B). They obtained products of hydroxylation at 4′ or both 3′ and 4′ positions in the B-ring. They have also observed that the distance between the carbonyl and hydroxyl groups in the A-ring has impact on the reaction speed and efficiency [15]. Herath and coworkers transformed 7-hydroxyflavone into 7,4′-dihydroxyflavone by means of the strain of A. alliaceus (ATCC 10060), whereas, using the strain of Beauveria bassiana (ATCC 7159) led to formation of 7-O-β-D-4-O-Methylglucopyranoside and 4′-hydroxyflavone 7-O-β-D-4-O-methylglucopyranoside. O-methylation of 7-hydroxyflavone was catalyzed by Nocardia species (NRRL 5646). The other substrate—3-hydroxyflavone—was transformed by means of B. bassiana (ATCC 13144) into 3,4′-dihydroxyflavone and flavone 3-O-β-D-4-O-methylglucopyranoside [16]. The same strain metabolized 5-hydroxyflavone into 5,4′-dihydroxyflavone and 4′-hydroxyflavone 5-O-β-D-4-O-methylglucopyranoside, whereas 6-hydroxyflavone was transformed into 6-hydroxyflavanone, flavone 3-O-β-D-4-O-methylglucopyranoside, and racemic flavanone 6-O-β-D-4-O-methylglucopyranoside [17].

The substrates that possess several hydroxyl groups in the flavonoid skeleton, like quercetin or fisetin, in the culture of S. griseus (ATCC 13273) underwent regioselective either hydroxylation or methylation [18]. In the other paper regioselective 4′-O-demethylation of tangeretin and 3-hydroxytangeretin catalyzed by A. niger was reported [19].

In this paper we present the study in which we used the filamentous fungi of genera Aspergillus and Penicillium to enzymatic transformation of a xenobiotic substrate (7-hydroxyflavanone). The aim of this research was to obtain new, not known in the literature, compounds with stronger antioxidant activity than the initial substrate, to establish the metabolic pathways of 7-hydroxyflavanone in filamentous fungi cultures and also to determine the reactions specific for the tested microorganisms.

2. Materials and Methods

2.1. Analysis

The course of microbial transformation was monitored by TLC (SiO2, DC Alufolien Kieselgel 60 F254, Merck, Darmstadt, Germany). Chromatograms were developed using the following developing systems: hexane : ethyl acetate (7 : 3), dichloromethane : ethyl acetate (1 : 1), toluene : diethyl ether (4 : 1). Column chromatography (SiO2, Kieselgel 60, 230–400 mesh, 40–63 μm, Merck) was performed using the same eluents. 1H NMR and 13C NMR spectra were recorded with a Bruker Avance DRX 300 spectrometer. IR spectra were determined with a Mattson IR 300 Thermo Nicolet spectrometer. Mass spectra were obtained using high-resolution electrospray ionization (ESI+-MS) (Waters LCT Premier XE mass spectrometer).

HPLC analyses were performed with a Waters 2690 instrument equipped with Waters 996 photodiode array detector, using ODS 2 column (4.6 × 250 mm, Waters) and a Guard-Pak Inserts μBondapak C18 pre-column. Separation conditions were as follows: gradient elution, using 80% of acetonitrile in 4.5% formic acid solution (eluent A) and 4.5% formic acid (eluent B); flow, 1 mL/min; detection wavelength 280 nm; program: 0–7 min, 10% A 90% B; 7–10 min, 50% A 50% B; 10–13 min, 60% A 40% B; 13–15 min, 70% A 30% B; 15–20 min 80% A 20% B; 20–30 min 90% A 10% B; 30–40 min, 100% A. Melting points were determined with a Boetius apparatus (Kofler block). Antioxidant properties were measured with a Cintra 20 spectrometer (GBC, Melbourne, Australia).

2.2. Materials

The racemic substrate for biotransformation—7-hydroxyflavanone (1)—was purchased from Aldrich.

2.2.1. 7-Hydroxyflavanone (1)

C15H12O3; melting point 188–190°C; 1H NMR see Table 1; 13C NMR see Table 2, IR (KBr, 𝜈 m a x , cm−1): 3413 (O–H, stretch), 1651 (C=O, stretch), 1577 (C–C, stretch, aromatic), 1260 (–C–OH, bending), and 1062 (–C–OH, stretch); HRESI-MS [M + H+] (calculated/found) (m/z 241.0865/241.0860).

tab1
Table 1: 1H NMR chemical shifts (δ) of compounds 1 ÷ 6.
tab2
Table 2: 13C NMR chemical shifts (δ) of compounds 1 ÷ 6.
2.2.2. Microorganisms

For our research we used a wild strain of A. niger KB and a UV mutant of A. niger (13/5). The KB strain was obtained from the collection of the Department of Biotechnology and Food Microbiology of Wrocław University of Environmental and Life Sciences (Poland) and the strain 13/5 from the University of Life Sciences in Lublin (Poland). The microorganisms were maintained on potato slants (sterilized piece of potato) at 5°C.

The wild strains A. ochraceus 456 and P. chermesinum 113 were obtained from the collection of the Department of Chemistry of Wrocław University of Environmental and Life Sciences (Poland). The microorganisms were maintained on agar slants at 5°C.

3. Biotransformations

3.1. Screening Procedure

Cultivation media consisted of 3% glucose (The Industrial and Trading Enterprise “Stanlab” Co. Ltd., Poland) and 1% peptobac (BTL sp. z o.o., Poland) in water. The microorganisms were transferred from the slants to 500 mL Erlenmayer flasks, each containing 200 mL of the medium. Preincubation was performed at 25°C for 24–48 h. Then portions of 1 mL of the culture solution were transferred to inoculate 500 mL flasks, each containing 200 mL of the medium. After cultivation at 25°C for 24 hours on a rotary shaker, 10 mg of a substrate, dissolved in 0.5 mL of THF, was added to the grown culture. Control cultivation with no substrate was also performed. After 3, 6 and 9 days of incubation under the above conditions, portions of 5 mL of the transformation mixture were withdrawn and extracted with ethyl acetate (3 × 3 mL). The extracts were dried over MgSO4 (5 min), concentrated in vacuo and analyzed by TLC. Quantitative analyses of the mixtures were performed by means of HPLC. Calibration curves for quantitative analyses were prepared using isolated and purified biotransformation products as standards.

3.2. Preparative Biotransformation

Portions of 1 mL of the Preincubation culture solution were used to inoculate three 2000 mL flasks, each containing 500 mL of the cultivation medium. The cultures were incubated at 25°C for 48 hours on a rotary shaker. Then 50 mg of the substrate dissolved in 2.5 mL of THF was added to each flask (100 mg of the substrate per 1 L of the cultivation mixture). After 3, 6, or 9 days of incubation the mixtures were extracted with ethyl acetate (3 × 200 mL), dried (MgSO4), and concentrated in vacuo. The transformation products were separated by column chromatography. Pure products were identified by means of spectral analyses (TLC, 1H NMR, 13C NMR, IR).

Physical and spectral data of the products obtained are presented next.

3.2.1. 7-Methoxyflavanone (2)

C16H14O3; melting point 89-90°C; 24% yield; purity 98% (HPLC); [ 𝛼 ] 2 0 5 4 6 = 0, ( 𝑐 = 0.95, CH3OH); 1H NMR see Table 1; 13C NMR see Table 2; IR (KBr, 𝜈 m a x , cm−1): 3032 (C–H stretch, aromatic), 1683 (C=O, stretch), 1614 (C–C, stretch, aromatic), and 1438 (C–C, stretch, aromatic); HRESI-MS [M + H+] (calculated/found) (m/z 255.1021/255.1009).

3.2.2. 3′,4′-Dihydroxy-7-methoxyflavanone (3)

C16H14O5; oily liquid; 19% yield; purity 98% (HPLC); [ 𝛼 ] 2 0 5 4 6 = 0, (c = 1.0, CH3OH); 1H NMR see Table 1; 13C NMR see Table 2; IR (KBr, 𝜈 m a x , cm−1): 3420 (O–H, stretch), 1710 (C=O, stretch), 1569 (C–C, stretch, aromatic), 1316 (C–O, stretch), and 1020 (C–O–C, stretch); HRESI-MS [M + H+] (calculated/found) (m/z 287.1022/287.1016).

3.2.3. 2,4-cis-7-Hydroxyflavan-4-ol (4)

C15H14O3; oily liquid; 74% yield; purity 98% (HPLC); [ 𝛼 ] 2 3 5 4 6 = 0, (c = 0.98, CH3OH), 1H NMR see Table 1; 13C NMR see Table 2; IR (CH2Cl2, 𝜈 m a x , cm−1): 3220 (O–H, stretch), 1621 (C–C, stretch, aromatic), and 1598 (C–C, stretch, aromatic); HRESI-MS [M + H+] (calculated/found) (m/z 243.1050/243.1043).

3.2.4. 2,4-trans-5,7-dihydroxyflavan-4-ol (5)

C15H14O4; melting point 275–277°C; 12% yield; purity 98% (HPLC); [ 𝛼 ] 2 0 5 4 6 = 0, (c = 1.95, CH3OH); 1H NMR see Table 1; 13C NMR see Table 2; IR (KBr, 𝜈 m a x , cm−1): 3460 (O–H, stretch), 1615 (C–C, stretch, aromatic), and 1590 (C–C, stretch, aromatic); HRESI-MS [M + H+] (calculated/found) (m/z 259.0923/259.0918).

3.2.5. 7-Hydroxyflavone (6)

C15H10O3; melting point 246–248°C; 98% yield; purity 99% (HPLC); 1H NMR see Table 1; 13C NMR see Table 2; IR (KBr, 𝜈 m a x , cm−1): 3412 (O–H, stretch), 2926 (O–H, stretch, intramolecular hydrogen bond), 1627 (C=O, stretch), 1454 (C–C, stretch, aromatic), and 773 (C–H, bending, aromatic); HRESI-MS [M + H+] (calculated/found) (m/z 239.0760/239.0755).

3.3. Measurement of Antioxidant Properties of the Substrate and the Products

A methanolic solution of DPPH (1,1-diphenyl-2-picryl-hydrazyl) of absorbance of about 1.00 was mixed with a proper amount of a tested flavonoid. After 20 min, disappearance of absorbance at 520 nm was measured. The initial concentration of DPPH was determined by means of calibration curve. The IC50 value (antiradical activity) was determined on the basis of graphs—DPPH radical reduction (expressed in %) as a function of concentration of the tested compound. IC50 means concentration of the antioxidant that reduces the initial concentration of DPPH by half.

4. Results and Discussion

At first screening tests on racemic 7-hydroxyflavanone (1) were performed, in which for catalysis we used enzymatic systems of twenty-seven microorganisms of Aspergillus, Penicillium, Coryneum, Nectria, Chaetomium, Absidia, Spicaria, and Cryptosporiopsis genera. The tests led to selection of four microorganisms capable of biotransformation of this substrate. The following microorganisms were selected: three strains of Aspergillus (a wild strain of A. niger KB, its UV-mutant-A. niger 13/5, and A. ochraceus 456) and the strain of Penicillium chermesinum 113.

A six-day transformation of 7-hydroxyflavanone (1), catalyzed by the strain of P. chermesinum 113, gave two products: 7-methoxyflavanone (2) in 24% yield and 3′,4′-dihydroxy-7-methoxyflavanone (3) in 19% yield (Scheme 1), with 8% of unreacted substrate 1 remained. Both the biotransformation products and the unreacted substrate were racemic.

254929.sch.001
Scheme 1

The structures of newly formed products: 7-methoxyflavanone (2) and 3′,4′-dihydroxy-7-methoxyflavanone (3), were determined by means of 1H NMR, 13C NMR, and IR. In the IR spectrum of product 2 there is no absorption band at 3413 cm−1, observed for the substrate (1), which means that there is no hydroxyl group in product 2. In the 1H NMR of 2 a new singlet at δ = 3.86 ppm appeared, integrating for 3 protons, which indicates the presence of a methoxyl group. The chemical shifts of A-ring protons did not considerably differ in the spectra of 1 and 2, which unequivocally suggest that O-methylation occurs at the C-7 position. The presence of the methoxyl group was also confirmed by 13C NMR of 2, where the signal at δ = 55.7 ppm is visible.

In the 1H NMR of 3′,4′-dihydroxy-7-methoxyflavanone (3) there are no considerable changes in the chemical shifts of H-5, H-6, and H-8 compared to the substrate (1); however, the presence of a methoxyl group at C-7 was confirmed by the singlet at δ = 3.84 ppm, integrating for three protons. Lack of H-3′ and H-4′ signals in the 1H NMR of 3 and the shift of C-3′ signal (from δ = 129.7 ppm to δ = 135.2 ppm) and C-4′ signal (from δ = 129.6 ppm to δ = 135.4 ppm) in the 13C NMR of 3 compared to the substrate confirms locations of the two hydroxyl groups at the C-3′ and C-4′ in the B-ring of product 3.

Microbial transformation of 7-hydroxyflavanone (1) by means of Aspergillus niger KB led to reduction of the carbonyl group. After 9 days of biotransformation 7-hydroxyflavan-4-ol (4) was isolated in 74% yield (Scheme 2). The reaction was continued until all the substrate was consumed. In the 1H NMR spectrum of 7-hydroxyflavan-4-ol (4) there is a doublet of doublets at δ = 5.03 ppm observed, integrating for one proton and ascribed to H-4 ( 𝐽 4 , 3 a x = 9.9 Hz, 𝐽 4 , 3 e q = 6.2 Hz). In the 13C NMR the signal of C-4 was shifted from δ = 193.1 ppm for 7-hydroxyflavanone (1) to δ = 65.6 ppm for 7-hydroxyflavan-4-ol (4). The chemical shifts of H-2, H-4, H-3ax, H-3eq protons and their coupling constants in the 1H NMR spectrum of 4 were compared with the spectra of flavan-4-ol and 6-hydroxyflavan-4-ol (published data). These confirmed the structure of 2,4-cis-7-hydroxyflavan-4-ol (4) [20]. Flavan-4-ol and 6-hydroxyflavan-4-ol were obtained in biotransformation of flavanone and 6-hydroxyflavanone, respectively, catalyzed by A. niger KB. The structure of flavan-4-one was determined by means of X-ray analysis [20].

254929.sch.002
Scheme 2

X-ray analysis of 2,4-cis-flavan-4-ol proved that the product was a racemic mixture of (2R, 4R) and (2S, 4S) isomers. The two crystallographically independent molecules have pseudoequatorial phenyl groups, whereas H-2 is in pseudoaxial position. This is confirmed by the values of the following angles: H-2–C-2–C-3–H3A = 67.11°; H-2–C-2–C-3–H3B = −174.21° and H2A–C2A–C3A–H3D = −67.80°; H2A–C2A–C3A–H3C = 173.04°, and also by the respective coupling constants: 𝐽 2 - 3 e q = 1.83 Hz and 𝐽 2 - 3 a x = 11.62 Hz (Table 3). In both molecules the 4-OH group is pseudo equatorial and 4-H is pseudo axial. This is confirmed by the respective coupling constants 𝐽 4 - 3 e q = 6.27 Hz and 𝐽 4 - 3 a x = 10.59 Hz (Table 3) as well as by the values of the angles: H-4–C-4–C-3–H3A = −46.61; H-4–C-4–C-3–H3B = −165.28 and H4A–C4A–C3A–H3D = 47.18; H4A–C4A–C3A–H3C = 166.34. (±)-7-Hydroxyflavan-4-ol (4) is an oily liquid; therefore it is not possible to carry out X-ray analysis of this compound. The 2,4-cis configuration of 4 was ascribed by comparing the NMR chemical shifts and coupling constants of H-2, H-4, H-3ax, H-3eq to the data for (±)-flavan-4-ol (Table 3).

tab3
Table 3: Selected 1H NMR data for 2,4-cis-7-hydroxyflavan-4-ol (4), 2,4-trans-5,7-dihydroxyflavan-4-ol (5), and 2,4-cis-flavan-4-ol.

The other product of biotransformation of 7-hydroxyflavanone (1) was found to be 5,7-dihydroxyflavan-4-ol (5). The reaction was catalyzed by the strain of A. ochraceus 456 (Scheme 2). After 3 days of transformation the product of hydroxylation at C-5 along with the carbonyl group reduction (5) was isolated in 12% yield as a racemic mixture. The reaction was stopped after 3 days, because longer biotransformation time resulted in degradation of both the unreacted substrate and the newly formed product 5. In the tenth day of the process there was no longer flavonoid structures observed in the reaction mixture.

Hydroxylation at C-5 was confirmed in the 1H NMR spectrum of 5 by the signals at δ = 7.40 ppm and δ = 7.42 ppm (two one-proton doublets of doublets with J = 2.12 Hz) ascribed to H-6 and H-8, and also in the 13C NMR where the signal at δ = 129.9 ppm observed in the spectrum of substrate 1 was shifted to δ = 159.7 ppm in the spectrum of product 5. In the 1H NMR of 5,7-dihydroxyflavan-4-ol (5) the presence of a one-proton doublet of doublets at δ = 5.52 ppm of H-4 indicates that the carbonyl group was reduced. This structural change was confirmed by the shift of the C-4 signal in the 13C NMR from δ = 193.1 ppm for the substrate to δ = 59.5 ppm for product 5. Comparison of coupling constants of 2,4-cis-7-hydroxyflavan-4-ol (4) and product 5 (Table 3) led us to the structure of 2,4-trans-5,7-dihydroxyflavan-4-ol, with both phenyl and hydroxyl groups in pseudoequatorial positions.

Transformation of 7-hydroxyflavanone (1) by means of A. niger 13/5 led to the product of C-2 and C-3 dehydrogenation (6) (Scheme 2). 7-Hydroxyflavone (6) after 9 days of biotransformation was isolated in 98% yield. The substrate was fully consumed.

In the 1H NMR of substrate 1 the signal of H-2 appears at δ = 5.43 ppm as a one-proton doublet of doublets with the coupling constants of J = 12.9 Hz and J = 3.0 Hz, whereas the signal of H-3ax is observed at δ = 2.96 ppm as a doublet of doublets integrating for one proton with J = 16.9 Hz and J = 12.8 Hz, and finally the signal of H-3eq is also a doublet of doublets at δ = 2.69 ppm with J = 16.9 Hz and J = 3.1 Hz. In the 1H NMR of the biotransformation product—7-hydroxyflavone (6)—there is no signal of H-2 and a new singlet of one proton appears at δ = 6.86 ppm, which is ascribed to the H-3. In the 13C NMR the signals of C-3 and C-2 are shifted from δ = 45.2 ppm and δ = 81.1 ppm for the substrate 1 to δ = 106.6 ppm (C-3) and δ = 162.8 ppm (C-2) for 7-hydroxyflavone (6). These changes unequivocally indicate the presence of a double bond between C-2 and C-3 in the C-ring of product 6.

Comparison of UV spectra of 7-hydroxyflavanone (1) and its biotransformation products shows that the most significant shift of the maximum absorption bands to the longer wavelengths occurs for 7-hydroxyflavone (6) (Table 4), where conjugation of two chromophores: the carbonyl group and the C=C double bond, results in delocalization of π and n electrons, which facilitate electron excitations associated with 𝑛 π* and ππ* transitions.

tab4
Table 4: UV absorption of 7-hydroxyflavanone (1) and its biotransformation products (2 ÷ 6).

For product 3 we observed an increase in absorption coefficient value compared to substrate 1 and a small bathochromic shift. In the case of the reduction products 4 and 5 a bathochromic shift of about Δ 𝜆 m a x = 8 nm (2nd band) is visible, which is slightly stronger for product 5, with the additional hydroxyl group at C-5 (Table 4). We suppose that a hydrogen bond that is formed between the hydroxyl groups at C-5 and C-4 in compound 5 leads to formation of a specific “complex” with different location of the molecular energy levels than in the case when there is no specific interactions of this kind. A part of energy of the molecule is used for hydrogen bond formation. This results in lowering of both ground and excited states. The smaller difference in energy between these states is responsible for the red shift in the electronic spectra (bathochromic effect).

For substrate 1 and the obtained biotransformation products (2–6) the IC50 values were measured in order to establish and to compare their antioxidant properties (Table 5) [21]. The four products of microbial transformation of 1 showed higher antioxidant properties than the initial substrate. The highest antioxidant activity was observed for 3′,4′-dihydroxy-7-methoxyflavanone (3) (IC50 = 6.70). Such high antioxidant properties of 3 arise from the presence of two hydroxyl groups in the B-ring. The two hydroxyl groups in orthoposition in product 3 may facilitate chelating of metal ions [2225].

tab5
Table 5: The IC50 values of the 7-hydroxyflavanone (1) and the biotransformation products.

5. Conclusions

( 1 ) Microbial transformations of 7-hydroxyflavanone (1) as well as the earlier study on biotransformation of flavanone and 6-hydroxyflavanone using the strains of genus Aspergillus allowed to determine the ability of these microorganisms to perform specific reaction types: ( i ) the strain of A. niger KB catalyzes reduction of the carbonyl group; ( i i ) the strain of A. niger 13/5 performs dehydrogenation of C-2 and C-3 positions of nonsubstituted flavanone and its monohydroxy derivatives. ( 2 ) The strain P. chermesinum 113, apart from its typical reaction of hydroxylation in the B-ring, performed O-methylation of 7-hydroxyflavanone (1). ( 3 ) A nontypical reaction of hydroxylation at C-5 along with carbonyl group reduction was observed for the strain A. ochraceus 456, which led to a new, not known in the literature, compound. ( 4 ) The highest yields of products were observed for 7-hydroxyflavan-4-ol (4) (74% yield) and 7-hydroxyflavone (6) (98% yield). The reactions were catalyzed by the strains A. niger KB and A. niger 13/5, respectively. ( 5 ) Microbial transformation of flavonoids may be used as a natural method leading to the derivatives of higher antioxidant properties than the initial substrates.

Conflict of Interests

The authors report no conflict of interests. The authors alone are responsible for the content and writing of the paper.

Acknowledgment

Publication/Project “Biotransformations for pharmaceutical and cosmetics industry” no. POIG.01.03.01-00-158/09 was part-financed by the European Union within the European Regional Development Fund.

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