The Use of Recombinant Pseudotype Virus-Like Particles Harbouring Inserted Target Antigen to Generate Antibodies against Cellular Marker p16INK4A
Figure 4
The reactivity of the MAbs raised against VP1/VP2-p16INK4A pseudotype VLPs with the p16INK4A protein sequence in Western blot. (a) Coomassie blue-stained SDS-PAGE. (b–d) Western blot with the MAbs raised against pseudotype VLPs, clones 9F12 (b), 11E12 (c), and 26D11 (d). Lane M: prestained protein molecular mass marker (Thermo Scientific Fermentas); lane 1: purified GST-fused p16INK4A protein; lane 2: purified pseudotype VP1/VP2-p16INK4A VLPs; lane 3: purified nonmodified VP1/VP2 VLPs; lane 4: crude lysate of nontransformed S. cerevisiae cells.
