Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions
Table 1
Genetic analysis of L. rhynchopetalum populations from the Bale mountains (BM) and Simien mountains (SM) at different hierarchical levels.
Population/group
Location-mountains
Altitude (m asl)
NGC /SS
NH
NPS
GD
ND
MIHD ± SD
Goba-1ad
15 km from Goba to Mena-BM
4000–4300
5
4
5
0.90 ± 0.16
0.071 ± −0.052
0.081 ± 0.045
2.99 ± 1.87
2.99 ± 2.19
2.40 ± 1.51
7.11 [1.54, 33.08]
1.12
Goba-2a
16.8 km from Goba to Mena-BM
4000–4300
5
1
0
0.00 ± 0.00
0.000 ± −0.000
0.000 ± 0.000
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 [0.00, 0.00]
0.00
Goba-3
18.5 km from Goba to Mena-BM
4000–4300
5
1
0
0.00 ± 0.00
0.000 ± 0.000
0.000 ± 0.000
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 [0.00, 0.00]
0.00
Debark-1bcdef
47 km from Debark to Sankaber-SM
3600–3800
5
5
6
1.00 ± 0.12
0.038 ± 0.031
0.042 ± 0.031
1.68 ± 1.17
1.68 ± 1.37
1.92 ± 1.27
—
−1.09
Debark-2b
48 km from Debark to Sankaber-SM
3600–3800
5
1
0
0.00 ± 0.00
0.000 ± 0.000
0.000 ± 0.000
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 [0.00, 0.00]
0.00
Debark-3be
51 km from Debark to Sankaber-SM
3600–3800
5
2
1
0.60 ± 0.17
0.014 ± 0.015
0.024 ± 0.024
0.61 ± 0.57
0.61 ± 0.67
0.48 ± 0.48
0.69 [0.15, 3.19]
1.22
Debark-4b
53 km from Debark to Sankaber-SM
3600–3800
5
2
2
0.40 ± 0.24
0.019 ± 0.019
0.048 ± 0.034
0.83 ± 0.70
0.83 ± 0.81
0.96 ± 0.76
0.69 [0.15, 3.18]
−0.97
Debark-5cf
54.5 km from Debark to Sankaber-SM
3600–3800
5
2
1
0.40 ± 0.24
0.009 ± 0.012
0.024 ± 0.024
0.41 ± 0.44
0.41 ± 0.52
0.48 ± 0.48
0.69 [0.15, 3.18]
−0.82
Bale
15
5
5
0.70 ± 0.08
0.035 ± 0.025
0.071 ± 0.042
1.48 ± 0.95
1.48 ± 1.06
1.54 ± 0.85
2.20 [0.77, 5.97]
−0.28
Simien
25
8
9
0.70 ± 0.08
0.025 ± 0.018
0.046 ± 0.032
1.11 ± 0.75
1.11 ± 0.83
1.85 ± 0.89
3.67 [1.57, 8.21]
−1.33
All
40
13
20
0.85 ± 0.04
0.28 ± 0.15
0.201 ± 0.062
8.23 ± 4.29
8.23 ± 4.32
3.99 ± 2.16
6.29 [3.19, 12.06]
0.81
NGC/SS: number of gene copies/sample size; NH: number of haplotypes; NPS: number of polymorphic sites; GD: gene diversity; ND: nucleotide diversity (based on polymorphic loci only); MIHD: mean interhaplotypic distance (based on Kimura 2P method); SD: standard deviation (for both the sampling and the stochastic processes); : mean number of pairwise difference (based on polymorphic loci only). Populations sharing the superscript a, b, or c have some haplotypes in common. Populations sharing the superscript d, e, or f were not significantly different from each other when tested using global test of population differentiation, based on Markov chain length of 10000 (significance level = 0.05). : Tajima’s test of selective neutrality. , , and are different estimators of theta (). *: Values in the square brackets are 95% confidence interval limits around . Note: for analysis at mountain-system level, all individuals of the same category were pooled together as a single unit.
