The Design of New Adjuvants for Mucosal Immunity to Neisseria meningitidis B in Nasally Primed Neonatal Mice for Adult Immune Response

Figure 1

(a)(1) SDS-PAGE (13% gel) analysis of native OMPs of N. meningitidis; (a)(2) LPS characterization and (a)(3) LPS immunoreactivity with monoclonal antibodies of N. meningitidis; (b) E. coli stx1 (1) or stx2 (2) toxoid after silver stain. Standard markers are shown on the left of (a); (c) immunoblot of 13% SDS/PAGE and transfer to nitrocellulose membranes. Nitrocellulose membranes containing OMPs of N. meningitidis (lanes (c)(1) and (c)(2)) were incubated with 1 : 500 dilution of rabbit antiserum anti-OMPs-Stx1 or anti-OMPs-Stx2, respectively. Immunoblot with 100 μg of toxoid Stx1 or Stx2; Lanes (d)(1) and (d)(2): IgG immunoreactivity of the monoclonal antibody anti-Stx1 or -Stx2, respectively, with the A subunit of the toxoid.