Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling

Figure 3

Experimental confirmation of one CODIS-STR locus identified by the Mixed Sequencer Reader. (a) The FBI CODIS Core STR Loci map (adapted from http://www.cstl.nist.gov/strbase/fbicore.htm). The D13S317 locus (chromosome 13:55144219-55144352 region) was randomly selected for analysis in this study. (b) The common repeat structure in the D13S317 locus in each person represents the combination of 2 structures in the CODIS database. (c) The direct sequencing chromatography trace of a PCR product containing the D13S317 locus at chromosome 13:55144219-55144352. (d) The MSR results indicated the presence of 8 and 9 copies of (TATC) within the individual. (e) The sequences of cloned PCR products were consistent with the MSR prediction. One of plasmid contained 8 copies of (TATC) (e.g., plasmid 1); whereas the other plasmids contained 9 copies of [TATC] (e.g., plasmid 2).